Leptospira Cultivation and Bioluminescent Strains

L. biflexa sevorar Patoc strain Patoc (Paris) and bioluminescent derivative PFlum7, L. interrogans Icterohaemorraghiae strain Verdun, L. interrogans serovar Copenhageni strain Fiocruz L1-130, L. interrogans serovar Manilae strain L495 and bioluminescent derivative strain MFLum1 were described earlier [7 (link), 9 (link), 10 (link)]. Bacteria were grown in Ellinghausen-McCullough-Johnson-Harris (EMJH) medium (Bio-Rad) at 28°C without agitation.

Free full text: Click here

Ratet G., Santecchia I., Fanton d’Andon M., Vernel-Pauillac F., Wheeler R., Lenormand P., Fischer F., Lechat P., Haake D.A., Picardeau M., Boneca I.G, & Werts C. (2017). LipL21 lipoprotein binding to peptidoglycan enables Leptospira interrogans to escape NOD1 and NOD2 recognition. PLoS Pathogens, 13(12), e1006725.

Publication 2017

EMJH) medium

Bacteria Strain

Corresponding Organization : VA Greater Los Angeles Healthcare System

Top 5 similar protocols

Variable analysis

independent variables

L. biflexa sevorar Patoc strain Patoc (Paris) and bioluminescent derivative PFlum7
L. interrogans Icterohaemorraghiae strain Verdun
L. interrogans serovar Copenhageni strain Fiocruz L1-130
L. interrogans serovar Manilae strain L495 and bioluminescent derivative strain MFLum1

dependent variables

Not explicitly mentioned

control variables

Bacteria were grown in Ellinghausen-McCullough-Johnson-Harris (EMJH) medium (Bio-Rad) at 28°C without agitation

controls

No positive or negative controls were explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!