PARG Inhibitor Effects on Colony Formation

Example 4

Colony formation assay was performed using HCC1937 (BRCA1-deficient breast cancer cells), HCC1937 BRCA1 (BRCA1-reconstituted HCC1937 cells) cells, PEO-1 (BRCA2-deficient ovarian cancer cells), and PEO-4 (BRCA2-reconstituted PEO-1 cells) with indicated concentrations of PARG inhibitor (#34).

Colony formation assay: HCC1937, HCC1937-BRCA1, PEO-1 or PEO-4 (˜1000 cells) were seeded into six-well plates and then treated by various doses of PARG inhibitor (#34). After 14-21 days of culture, the viable cells were fixed by methanol and stained with crystal violet. The number of colonies (>50 cells for each colony) was calculated.