Characterization of Silver Nanoparticles

The size and structure of AgNPs were studied from images obtained using a transmission electron microscope (TEM) Tecnai G2 F20 X-TWIN (FEI, Lausanne, Switzerland), equipped with a field emission electron gun at the accelerating voltage of 200 kV. For a microscopy study, the diluted samples were deposited on the TEM grids. AgNP diameter was measured with software ImageJ-win32 [7 (link),29 (link),30 (link)]. In addition, the microstructure of the sample was analyzed using a Scanning Electron Microscope SEM FEI Quanta 200 FEG (FEI, Hillsboro, OR, USA). The plant and lichen samples were examined in a low-vacuum mode operating at 20.0 kV using an LDF detector.

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Balčiūnaitienė A., Štreimikytė P., Puzerytė V., Viškelis J., Štreimikytė-Mockeliūnė Ž., Maželienė Ž., Sakalauskienė V, & Viškelis P. (2022). Antimicrobial Activities against Opportunistic Pathogenic Bacteria Using Green Synthesized Silver Nanoparticles in Plant and Lichen Enzyme-Assisted Extracts. Plants, 11(14), 1833.

Publication 2022

Tecnai G2 F20 X-TWIN FEI Quanta 200 FEG

Electron Lichen Microscopy Plant Scanning electron microscope Transmission electron microscope Twin Vacuum

Corresponding Organization : Lithuanian Research Centre for Agriculture and Forestry

Other organizations : Lithuanian University of Health Sciences

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Variable analysis

independent variables

Transmission electron microscope (TEM) Tecnai G2 F20 X-TWIN (FEI, Lausanne, Switzerland), equipped with a field emission electron gun
Accelerating voltage of 200 kV

dependent variables

Size and structure of AgNPs
AgNP diameter

control variables

Diluted samples deposited on the TEM grids
Scanning Electron Microscope SEM FEI Quanta 200 FEG (FEI, Hillsboro, OR, USA) used in low-vacuum mode operating at 20.0 kV using an LDF detector for examining plant and lichen samples

controls

No positive or negative controls were explicitly mentioned.

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