Single-cell Isolation and Characterization of Hair Bulge Cells

Single-cell suspensions were prepared from vibrissa hair bulge from the back skin
of experimental mice, according to a previously established method (Takeo et al., 2021 (link)). Cells were then
resuspended in PBS containing 0.5% BSA, and stained with FITC anti-mouse CD34
antibody (Thermo Fisher Scientific) and PE anti-mouse CD49f antibody (Thermo
Fisher Scientific) for 15 min at 4°C. After staining, cells were analyzed
by flow cytometry using BD FACSLyric^TM (BD Biosciences, Mountain
View, CA, USA) with BD FlowJo^TM software (BD Biosciences).

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Song M., Shim J, & Song K. (2024). Oral Administration of Lactilactobacillus curvatus LB-P9 Promotes Hair Regeneration in Mice. Food Science of Animal Resources, 44(1), 204-215.

Publication 2024

BD FACSLyricTM

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Variable analysis

independent variables

Vibrissa hair bulge from the back skin of experimental mice

dependent variables

Fluorescence intensity of FITC anti-mouse CD34 antibody
Fluorescence intensity of PE anti-mouse CD49f antibody

control variables

Cell suspension preparation method
Staining conditions (PBS containing 0.5% BSA, 15 min at 4°C)
Flow cytometry analysis (BD FACSLyric, BD FlowJo software)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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