Live imaging was performed as described previously (Geiger et al., 2011 (link); Carvalho et al., 2014 (link)). Dechorionated stage 15 embryos were mounted on their ventral side on glass-bottomed culture dishes (MatTek) with embryo glue (double-sided tape diluted in heptane) and covered with halocarbon oil 27 (Sigma-Aldrich). Embryos were wounded as described above for the wounding assay except that the laser power was lower in order to inflict smaller wounds that are able to close during the imaging procedure. Time-lapse microscopy of transgenic embryos was performed at 25°C on a Nikon/Andor Revolution XD spinning-disk confocal microscope with an EMCCD camera (iXon 897) using iQ software and using a 60× Plan Apochromat VC PFS 1.4 NA oil-immersion objective, a 60× Plan Apochromat VC PFS 1.2 NA water-immersion objective, or a 100× Plan Apochromat PFS 1.4 NA oil-immersion objective (Nikon). Individual z slices with a step size of 0.36 µm (60× objectives) or 0.25 µm (100× objectives) were acquired every 1, 2.5, or 5 min for 60–300 min.