Quercetin Modulates Lipid Metabolism

Quercetin (purity ≥95%, high performance liquid chromatography) purchased from Sigma-Aldrich Co. (St Louis, MO, USA) was dissolved in DMSO and the dosages of Quercetin chosen were based on previous reports.23 (link),25 (link),26 (link) Commercial kits for urine albumin, urine creatinine, blood urea nitrogen, and serum creatinine were purchased from Jiancheng Biotechnology Company (Nanjing, China). Total cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) kits were purchased from Shanghai Roche (Shanghai, China). Total protein extraction kit and bicinchoninic acid protein assay kit were provided by Jiangsu KeyGEN BioTECH Corp., Ltd. (Nanjing, China). Antibodies including HMGCR, LDLr, SREBP-2, SCAP, and β-actin were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Other chemicals were of analytical grade and supplied by local reagent retailer.

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Jiang X., Yu J., Wang X., Ge J, & Li N. (2019). Quercetin improves lipid metabolism via SCAP-SREBP2-LDLr signaling pathway in early stage diabetic nephropathy. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 12, 827-839.

Publication 2019

Quercetin Total protein extraction kit bicinchoninic acid protein assay kit HMGCR SREBP-2 β-actin

Actin Albumin Antibodies Assay Bicinchoninic acid Blood urea nitrogen Cholesterol Creatinine Dmso High density lipoprotein cholesterol High performance liquid chromatography Hmgcr Ldlr Low density lipoprotein cholesterol Protein Quercetin Serum Triglycerides Urine

Corresponding Organization : Nanjing University of Chinese Medicine

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Variable analysis

independent variables

Quercetin (purity ≥95%, high performance liquid chromatography) purchased from Sigma-Aldrich Co. (St Louis, MO, USA)

dependent variables

Urine albumin
Urine creatinine
Blood urea nitrogen
Serum creatinine
Total cholesterol
Triglycerides
Low-density lipoprotein cholesterol (LDL-C)
High-density lipoprotein cholesterol (HDL-C)

control variables

Dosages of quercetin chosen were based on previous reports

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