Cell RALDH1 activity was determined by using the ALDEFLUOR staining kit (STEMCELL Technologies Inc.) (5 (link)). Briefly, cells were resuspended at 106 cells/ml in ALDEFLUOR assay buffer, containing activated ALDEFLUOR substrate with or without the inhibitor DEAB, and incubated at 37°C for 30 min. ALDEFLUOR-reactive cells were detected in the FITC channel.
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ALDEFLUOR Assay for RALDH1 Activity
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Corresponding Organization : The University of Texas Medical Branch at Galveston
Other organizations : Xiangya Hospital Central South University, Central South University
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Variable analysis
independent variables
- Presence or absence of the inhibitor DEAB
- ALDEFLUOR-reactive cells detected in the FITC channel
- Cell resuspension at 10^6 cells/ml in ALDEFLUOR assay buffer
- Incubation at 37°C for 30 minutes
- Positive control: Cells incubated with activated ALDEFLUOR substrate
- Negative control: Cells incubated with activated ALDEFLUOR substrate and the inhibitor DEAB
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