Measuring Lactate Dehydrogenase Activity

The activity of lactate dehydrogenase (LDH) was measured using a lactate dehydrogenase activity assay kit (Sigma) as described earlier [16 (link),17 (link),18 (link)]. A volume from the MTT assay was used and plated in a 96-well plate. An LDH master mix was prepared and added to each well. The reaction was carried out at room temperature in the dark. The resultant absorbance was measured at 450 nm with the Thermo-Fisher Multiskan MCC plate reader (Fisher).

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Sheinin M., Jeong B., Paidi R.K, & Pahan K. (2022). Regression of Lung Cancer in Mice by Intranasal Administration of SARS-CoV-2 Spike S1. Cancers, 14(22), 5648.

Publication 2022

lactate dehydrogenase activity assay kit Multiskan MCC plate reader

Assay Lactate dehydrogenase

Corresponding Organization : Jesse Brown VA Medical Center

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Activity of lactate dehydrogenase (LDH)

control variables

None explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

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