The liposome formulation combined chloroform dissolved samples of cholesterol, DOPE, DSPC, DSPE-(PEO)4-cRGDfK, DSPE-mPEG2000, and DiO (6:6:6:1:1:0.5 molar ratio). The chloroform was evaporated under argon gas and the dried lipid film was hydrated with a solution containing 10 mg of human serum albumin in 1 ml phosphate buffer pH 7.4 for a minimum of 30 minutes. The solution was vortexed for 1 minute to remove any adhering lipid film and sonicated in a bath sonicator (ULTRAsonik 28X) for 1 minute at room temperature to produce multilamellar vesicles. These vesicles were then sonicated with a Ti-probe (Branson 450 sonifier) for 2 minutes to produce small unilamellar nanoliposomes filled with the cross-linked human serum albumin gel as indicated by the formation of a translucent solution. To reduce the size of the filled nanoliposomes, stepwise extrusion was carried out with the final step being extrusion through a polycarbonate filter with 200-nm pore size (Whatman). The nanoliposomes are then purified by size exclusion chromatography on sepharose CL-4B columns to remove free albumin. The final concentration, size distribution, and zeta potentials were the same as the empty nanoliposomes described in section 2.3.