SERS measurements were conducted using a home-built, inverted high-speed Raman confocal microscope (Figure S5 ) equipped with a compact LM series volume holographic grating-stabilized 785 nm near-infrared diode laser, a 60× oil immersion objective lens with a numerical aperture of 0.65–1.25 (RMS60X-PFOD, Olympus), a HoloSpec f/1.8 spectrograph and an iDus CCD Camera. The detailed information related to the set-up can be found in our previous work.24 (link)–27 The SERS measurements were acquired at room temperature with 100 ms integration time and a laser power of 5 mW at the sample, unless otherwise mentioned.
UV-vis extinction spectra were collected on an Aviv Model 14DS UV-vis spectrophotometer (Aviv Biomedical, Lakewood, NJ). qPCR was performed on a CFX96 Touch real-time PCR detection system (Bio-Rad, Hercules, CA). Transmission electron microscopic (TEM) images were taken with an FEI Tecnai T12 microscope (FEI, Hillsboro, OR) at an accelerating voltage of 120 kV. The air-dried samples were prepared through deposition of a drop of samples onto the TEM 200-mesh copper grid (Ted Pella, Redding, CA).
UV-vis extinction spectra were collected on an Aviv Model 14DS UV-vis spectrophotometer (Aviv Biomedical, Lakewood, NJ). qPCR was performed on a CFX96 Touch real-time PCR detection system (Bio-Rad, Hercules, CA). Transmission electron microscopic (TEM) images were taken with an FEI Tecnai T12 microscope (FEI, Hillsboro, OR) at an accelerating voltage of 120 kV. The air-dried samples were prepared through deposition of a drop of samples onto the TEM 200-mesh copper grid (Ted Pella, Redding, CA).
