In an Erlenmeyer conical flask, one kilogram of the coarsely powdered root was macerated in 80% methanol (1:6 ratio)23 for three days with regular shaking. After three days, the mixture was filtered with sterile gauze followed by Whatman filters paper No. 1. To increase the extract yield, the residue was again macerated twice with the same amount of methanol for a total of six days. The mixed filtrates were evaporated and concentrated by a rotatory evaporator and a drying oven at 40°C, respectively. Finally, the concentrated extract was frozen in a deep freezer and then freeze dried in a lyophilizer to remove the water from the extract. The final dried extract (131.20 gm) was stored in a refrigerator at −4°C until required.24 (link),25 (link)