A FLIR E6 infrared thermal camera (FLIR Systems, Wilsonville, OR) was used to measure surface body temperature (14 (link)). After mice were removed from nests, the surface temperature was immediately obtained using a double-blind protocol. During capture of thermal images, animal behavior and scanning distance were controlled to avoid artificial effects. For reduction of variances, rectal body temperature was also measured using a highly precise electronic thermometer (TH-5 Thermalert Clinical Monitoring Thermometer; Physitemp Instruments).
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Infrared Thermal Imaging for Surface Temperature
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Corresponding Organization : Washington State University
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Variable analysis
independent variables
- Mice were removed from nests
- Surface body temperature
- Rectal body temperature
- Animal behavior during thermal image capture
- Scanning distance during thermal image capture
- Double-blind protocol for measuring surface temperature
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