Cardiometabolic Biomarker Profiling

For the subset of the original population that underwent subsequent cardiometabolic testing (n = 42), our health care provider team performed a physical examination on each of these patients and obtained each patient’s demographic data, clinical history, and anthropometric measurements. Blood samples were collected after an overnight fast and analyzed for basic chemistry, complete lipid profile, and high sensitivity C-reactive protein at the NIH Clinical Center. Interleukins (IL)-1β, IL-6, IL-18 were measured using a multiplex ELISA (Meso Scale Diagnostics, Rockville MD, USA), as described previously (Bastarache, Koyama, Wickersham, & Ware, 2014 (link)).