Rotenone (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in 4% carboxymethylcellulose (CMC, Sigma-Aldrich) with 1.25% chloroform (Beijing Chemical Works, Beijing, China). The fresh Rotenone solution was orally administrated (30 mg/kg body weight) by gavage once a day for 4 weeks [32 (link), 38 (link)].
For FMT administration, fresh stools were collected from the control group mice, and 600 mg of feces were immediately placed into 6 mL of sterile saline and were steeped for 1 min. Then, the dissolved feces were centrifuged at 1000 g (4 °C) for 3 min. The suspension was collected and 100 μL of bacterial suspension was then delivered to each recipient mouse via oral gavage within 10 min.
For FMT administration, fresh stools were collected from the control group mice, and 600 mg of feces were immediately placed into 6 mL of sterile saline and were steeped for 1 min. Then, the dissolved feces were centrifuged at 1000 g (4 °C) for 3 min. The suspension was collected and 100 μL of bacterial suspension was then delivered to each recipient mouse via oral gavage within 10 min.
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