Maintaining Isofemale Colonies for Malaria Research

Both IndCh and IndInt isofemale lines were maintained as two separate isofemale colonies after 20 generations and 23 generations, respectively, instead of single female progeny [29 ]. These two lines are currently being maintained in TIGS insectary. In both the colonies, males and females were allowed to inbreed within the filial generation, blood-fed and allowed to lay eggs in masses. All the key fitness parameters were monitored and recorded. These two isofemale colonies were utilized for insecticide-susceptibility assays and vectorial competence studies with Plasmodium berghei and Plasmodium falciparum.