Biomarkers of Intervertebral Disc Degeneration

We determined the levels of TNF‐α, IL‐1β, IL‐6, matrix metalloproteinase (MMP)‐3, MMP‐13, Collagen II, and aggrecan in the serum and vertebral disc tissue. We used TNF‐α (RTA00, Bio‐Techne, Minneapolis, Minnesota, USA), IL‐1β (RLB00, Bio‐Techne, Minneapolis, Minnesota, USA), IL‐6 (437107, BioLegend, San Diego, CA, USA), MMP‐3 (SEA101Ra, Cloud‐Clone Corp, Houston, TX, USA), MMP‐13 (SEA099Ra, Cloud‐Clone Corp, Houston, TX, USA), Collagen II (ER0852, Wuhan Fine Biotech, Wuhan, China), and aggrecan (SEB908Ra, Cloud‐Clone Corp, Houston, TX, USA) Enzyme‐Linked Immunosorbent Assay (ELISA) kits to measure relative factors.

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Yao B., Cai Y., Wang W., Deng J., Zhao L., Han Z, & Wan L. (2023). The Effect of Gut Microbiota on the Progression of Intervertebral Disc Degeneration. Orthopaedic Surgery, 15(3), 858-867.

Publication 2023

Aggrecan Clone Collagen Enzyme linked immunosorbent assay Il 1β Matrix metalloproteinase 13 Matrix metalloproteinase 3 Serum Tissue Tnf α Vertebral

Corresponding Organization : Hunan Children's Hospital

Other organizations : Third Xiangya Hospital, Central South University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Levels of TNF‐α
Levels of IL‐1β
Levels of IL‐6
Levels of matrix metalloproteinase (MMP)‐3
Levels of MMP‐13
Levels of Collagen II
Levels of aggrecan

control variables

None explicitly mentioned

positive controls

No positive controls were specified

negative controls

No negative controls were specified

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