Uveal melanoma spheroids were prepared and placed in a collagen matrix as previously described (Smalley, Contractor, et al., 2008a (link); Smalley et al., 2006 (link)). The spheroids were treated with drugs for 72 hr, and subsequently stained with Live/Dead viability stain (Thermo Fisher, Carlsbad, CA) to be analyzed using a Nikon-300 inverted fluorescence microscope (Nikon, Melville, NY). Red puncta, indicating dead cells, were measured for each spheroid.
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Uveal Melanoma Spheroid Viability Assay
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Corresponding Organization : Moffitt Cancer Center
Other organizations : Universidade Federal de Ciências da Saúde de Porto Alegre, Thomas Jefferson University, Sylvester Comprehensive Cancer Center, University of Miami, University of Florida Health
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Variable analysis
independent variables
- Drugs
- Red puncta (indicating dead cells)
- Cell viability
- Collagen matrix
- Duration of drug treatment (72 hr)
- Live/Dead viability staining
- Nikon-300 inverted fluorescence microscope
- Not specified
- Not specified
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