DHEA-Induced Polycystic Ovary Syndrome in Rats

Forty-eight SD rats (PCOS group, n=48) were given subcutaneous injection of 60 mg/kg/d dehydroepiandrosterone (DHEA; APExBIO Technology LLC, Houston, USA) diluted with 0.2 ml soybean for 35 consecutive days [19 (link), 26 (link)]. The remaining rats (SDR group, n=10) were subcutaneously injected by 0.2 ml soybean oil (Shanghai Yuanye Biological Technology Co., LTD, Shanghai, China) per day. PCOS rats model was successfully established when irregular estrus cycle happened on rats. Ten PCOS rats were treated as PCOSR group (n=10), and thirty PCOS rats were divided into three groups (n=10) being administrated by gavage with Paeoniflorin (Chengdu Herbpurify Co., Ltd, Chengdu, China) on 20 mg/kg/d, 40 mg/kg/d, 80 mg/kg/d respectively [12 (link)]. Rats in SDR and PCOSR groups were treated with the same weight-based volume of distilled water by gavage for 4 weeks. All animals were euthanized under ether narcotization, and the specimens were collected for the following examinations.

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Zhou J., Tan Y., Wang X, & Zhu M. (2021). Paeoniflorin attenuates DHEA-induced polycystic ovary syndrome via inactivation of TGF-β1/Smads signaling pathway in vivo. Aging (Albany NY), 13(5), 7084-7095.

Publication 2021

Paeoniflorin

Animals Biological Dhea Estrus Ether Examinations Gavage Paeoniflorin Pcos Rats Soybean Soybean oil Subcutaneous injection

Corresponding Organization :

Other organizations : Nanjing University of Chinese Medicine, Second People's Hospital of NanTong, Nantong University

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Variable analysis

independent variables

Subcutaneous injection of 60 mg/kg/d dehydroepiandrosterone (DHEA) for 35 consecutive days
Subcutaneous injection of 0.2 ml soybean oil per day
Oral administration of Paeoniflorin at 20 mg/kg/d, 40 mg/kg/d, and 80 mg/kg/d for 4 weeks

dependent variables

Irregular estrus cycle in PCOS rats
Unspecified outcome measures

control variables

Rat strain (SD rats)
Weight-based volume of distilled water administered by gavage for 4 weeks in SDR and PCOSR groups

positive control

SDR group (n=10) receiving subcutaneous injection of 0.2 ml soybean oil per day

negative control

PCOSR group (n=10) receiving weight-based volume of distilled water by gavage for 4 weeks

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