Orthopyridyl disulfide functionalized polyethylene glycol
N-hydroxysuccinimide ester (OPSS-PEG-NHS, MW 5000) was purchased from Nanocs,
Inc. (New York, NY). Trastuzumab (Herceptin®) was a generous gift from
Dr. Virginia Borges (UC Denver, CO, USA). Listeriolysin O (LLO) from E.
coli transfected with the LLO-pEt29-DP-E3570 plasmid, kindly
provided by Dr. Dan Portnoy (UC Berkeley, CA, USA), was purified by the method
described previously (13 (link),14 (link)) and stored in storage buffer (50 mM phosphate
buffer, pH 6.0, 1 M NaCl, 1 mM EDTA) without dithiothreitol (DTT) to preserve
its activity. Polylysine hydrobromide (MW 37,000; degree of polymerization: 177)
and 2-iminothiolane-HCl (Traut's reagent) were purchased from Sigma Life
Science (St. Louis, MO, USA). CL-4B Sepharose used for the purification of the
one-component complexes was purchased from Amersham Biosciences (Uppsala,
Sweden). All other reagents, unless otherwise specified, were purchased from
Thermo Fisher Scientific (Pittsburgh, PA, USA).
N-hydroxysuccinimide ester (OPSS-PEG-NHS, MW 5000) was purchased from Nanocs,
Inc. (New York, NY). Trastuzumab (Herceptin®) was a generous gift from
Dr. Virginia Borges (UC Denver, CO, USA). Listeriolysin O (LLO) from E.
coli transfected with the LLO-pEt29-DP-E3570 plasmid, kindly
provided by Dr. Dan Portnoy (UC Berkeley, CA, USA), was purified by the method
described previously (13 (link),14 (link)) and stored in storage buffer (50 mM phosphate
buffer, pH 6.0, 1 M NaCl, 1 mM EDTA) without dithiothreitol (DTT) to preserve
its activity. Polylysine hydrobromide (MW 37,000; degree of polymerization: 177)
and 2-iminothiolane-HCl (Traut's reagent) were purchased from Sigma Life
Science (St. Louis, MO, USA). CL-4B Sepharose used for the purification of the
one-component complexes was purchased from Amersham Biosciences (Uppsala,
Sweden). All other reagents, unless otherwise specified, were purchased from
Thermo Fisher Scientific (Pittsburgh, PA, USA).
