Immunohistochemical expression of GRP78 was confirmed by 1-& 2-D-WB using the same antibodies mentioned above and similarly reported earlier [23 (link),24 (link)]. Two-dimensional gel electrophoresis was performed as reported previously [25 (link)]. Proteins were separated and transferred to a nitrocellulose membrane [26 (link)], and immunoreactive GRP78 protein on the membrane was determined as reported earlier [19 (link)].
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