Propagation and Titration of West Nile Virus

BHK21 and L929 cells were maintained in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 2mM L-glutamine, 1 mM sodium pyruvate, antibiotic-antimycotic solution, and 1× nonessential amino acids (complete DMEM). WNV strain TX 2002-HC (WN-TX) was isolated by as previously described [11] (link). Working stocks of WN-TX were generated by a single round of amplification on Vero-E6 (ccl-81; ATCC) cells, and supernatants were collected, aliquoted, and stored at −80°C. Virus stocks were titered by a standard plaque assay on BHK21 cells as previously described [40] (link).

Free full text: Click here

Suthar M.S., Ma D.Y., Thomas S., Lund J.M., Zhang N., Daffis S., Rudensky A.Y., Bevan M.J., Clark E.A., Kaja M.K., Diamond M.S, & Gale M J.r. (2010). IPS-1 Is Essential for the Control of West Nile Virus Infection and Immunity. PLoS Pathogens, 6(2), e1000757.

Publication 2010

Amino acids Antibiotic Assay Cells Eagle Fetal bovine serum Glutamine L929 cells Plaque Pyruvate Sodium Strain Virus

Corresponding Organization : University of Washington

Other organizations : Washington University in St. Louis

Top 5 similar protocols

Protocol cited in 12 other protocols

Variable analysis

independent variables

WNV strain TX 2002-HC (WN-TX)

dependent variables

Virus titers

control variables

BHK21 cells
L929 cells
Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 2mM L-glutamine, 1 mM sodium pyruvate, antibiotic-antimycotic solution, and 1× nonessential amino acids (complete DMEM)
Vero-E6 (ccl-81; ATCC) cells

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!