Biodistribution and Clearance of CuNCs

Biodistribution and clearance studies were performed using female wild type C57BL/6 mice (Charles River Laboratory, Wilmington, MA), as previously reported.^{26 (link)} Typically, ⁶⁴Cu-CuNCs or ⁶⁴Cu-CuNCs-FC131 (approximately 740 kBq/100 μL saline (APP pharmaceuticals, Schaumburg, IL)) was injected into mice anesthetized with inhaled isoflurane through tail vein. At 1, 4, 24 and 48 h post injection, mice were euthanatized by cervical dislocation (n = 4 / time point) and organs of interest were collected, weighed, and counted in a Beckman 8000 gamma counter (Beckman, Fullterton, CA). To measure the clearance of the two nanoclusters, a group of mice (n=4) was housed in a metabolism study cage to collect urine and feces at 4, 24, and 48 h post injection. Standards for each nanocluster were prepared and counted with the biodistribution samples together to calculate percentage of the injected dose per gram of tissue (%ID g⁻¹).