Characterization of KSHV Latent Gene Regulation

SLK (NIH AIDS Reagent Program) and 293T (ATCC) cells were maintained in DMEM medium supplemented with 10% FBS, and penicillin/streptomycin (P/S). BCBL-1 (NIH AIDS Reagent Program) was cultured in RPMI medium with 10% FBS and P/S. iSLK cells carrying BAC16 mutants were grown in DMEM medium with 10% FBS, P/S, 1 μg/ml puromycin, 250 μg/ml G418 and 1 mg/ml hygromycin. The origin of iSLK cell line was previously described [55 (link)]. The following antibodies were used in ChIPs and immunoblots: anti-histone H3 (Abcam ab1791), anti-H3K27me3 (Active Motif #39155), anti-H3K4me3 (Active Motif #39159), anti-EZH2 (Active Motif #39875), anti-SUZ12 (Active Motif #39357), anti-RING1B (Abcam ab3832), anti-BMI1 (Abcam ab14389), LANA (Advanced Biotechnologies #13-210-100). Anti-SPT5 and anti-CyclinT1 antibodies were purchased from Santa Cruz Biotechnology.