Small-Angle X-Ray Scattering of Protein-RNA Complexes

SAXS data were collected in batch mode on the EMBL/DESY synchrotron beamline P12 [52 (link)], using standard procedures [53 (link)]. In addition to LigT alone, yeast tRNA (Sigma) and a 1:1 molar mixture of LigT and tRNA were analyzed. Data were processed with the ATSAS package [54 (link)]. Distance distribution functions were analyzed using GNOM [55 ]. For modelling LigT alone, GASBOR [56 (link)] was used. For modelling the protein-RNA complex, we used the program MONSA [57 (link)] with the protein and RNA in different phases. The tRNA alone was modelled using DAMMIN [57 (link)]. Molecular weight was estimated through a comparison of the forward scattering intensity of the sample to that of a fresh sample of monomeric bovine serum albumin.

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Myllykoski M, & Kursula P. (2017). Structural aspects of nucleotide ligand binding by a bacterial 2H phosphoesterase. PLoS ONE, 12(1), e0170355.

Publication 2017

yeast tRNA

Bovine serum albumin Molar Protein Trna Yeast

Corresponding Organization : University of Bergen

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Variable analysis

independent variables

Samples analyzed: LigT alone, yeast tRNA (Sigma), and a 1:1 molar mixture of LigT and tRNA

dependent variables

SAXS data collected
Distance distribution functions analyzed using GNOM
Modelling of LigT alone using GASBOR
Modelling of protein-RNA complex using MONSA
Modelling of tRNA alone using DAMMIN
Molecular weight estimated through comparison to bovine serum albumin

control variables

SAXS data collected on EMBL/DESY synchrotron beamline P12 using standard procedures
Data processed with the ATSAS package

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