Fucoidan nanoparticles synthesised by the polyelectrolyte complexation method were characterised by FTIR and 1 H NMR. FTIR-ATR spectroscopy was carried out to characterise the Fu-PEI complex using the FTIR-ATR model IR Prestige-21 Shimadzu spectrophotometer. The spectra of fucoidan, PEI and FuNPs were recorded at 4 cm -1 resolution with 20 scans. The absorbance bands were measured as a function of wave numbers between 400 and 4000 cm -1 . Pure fucoidan and the fucoidan-PEI complex were dissolved in methanol-d 4 , and 1 H NMR spectra were recorded using a Bruker AMX500 spectrometer. The chemical shifts δ are given in ppm and referenced to the external standard TMS or an internal solvent standard. Crystallinity and thermal stability of fucoidan nanoparticles were evaluated by XRD and thermogravimetric analysis, respectively.
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Characterization of Fucoidan Nanoparticles
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Other organizations : Cochin University of Science and Technology, Rajiv Gandhi Centre for Biotechnology
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Variable analysis
independent variables
- Synthesis method (polyelectrolyte complexation)
- Characterization of fucoidan nanoparticles (FuNPs)
- FTIR-ATR spectroscopy
- 1H NMR spectroscopy
- Crystallinity
- Thermal stability
- Resolution of FTIR-ATR (4 cm-1)
- Number of scans for FTIR-ATR (20)
- Solvent for 1H NMR (methanol-d4)
- External standard for 1H NMR (TMS)
- Positive control: Pure fucoidan
- Positive control: Fucoidan-PEI complex
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