Retinal Preparation for Electrophysiology

Mice retinae were prepared as previously described (Lefebvre et al., 2008 (link)). Briefly, mice were euthanized and retinae dissected out under infrared illumination to preserve dark adaptation. As much vitreous humor was removed as practical. A piece of retina was placed with ganglion cells facing down on an MSEA on the stage of an upright microscope. The preparation was continuously superfused with Ames' medium (A1420; Sigma Aldrich, St Louis, MO) equilibrated with 95% O₂ and 5% CO₂ gas at room temperature.

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Nagarah J.M., Stowasser A., Parker R.L., Asari H, & Wagenaar D.A. (2015). Optically transparent multi-suction electrode arrays. Frontiers in Neuroscience, 9, 384.

Publication 2015

A1420

Ames Dark adaptation Ganglion Infrared illumination Mice Microscope Placed cells Retina Vitreous humor

Corresponding Organization : University of Cincinnati

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Electrophysiological recordings from retinal ganglion cells

control variables

Mice were euthanized
Retinae were dissected out under infrared illumination to preserve dark adaptation
Retinal piece was placed with ganglion cells facing down on an MSEA
The preparation was continuously superfused with Ames' medium equilibrated with 95% O2 and 5% CO2 gas at room temperature

controls

No positive or negative controls were explicitly mentioned

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