Protocol detail

Flagellin Quantification Protocol

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Flagellin levels were determined as described previously^{41 (link)}. The bacterial suspension washed from the LB agar plate with PBS (10 ml, OD_600nm = 0.6) was used to prepare flagellin by vigorous vortexing and subsequent centrifugation. Flagellin in the supernatant was precipitated with trichloroacetic acid (5%, w/v), separated on 12% SDS-PAGE and stained with Coomassie Brilliant Blue R (Sigma-Aldrich B7920).

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Tsai Y.L., Chien H.F., Huang K.T., Lin W.Y, & Liaw S.J. (2017). cAMP receptor protein regulates mouse colonization, motility, fimbria-mediated adhesion, and stress tolerance in uropathogenic Proteus mirabilis. Scientific Reports, 7, 7282.

Publication 2017

Coomassie Brilliant Blue R

Agar Bacterial Centrifugation Coomassie brilliant blue r Flagellin Sds page Trichloroacetic acid

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Variable analysis

independent variables

Flagellin levels

dependent variables

Flagellin levels in the supernatant

control variables

Bacterial suspension washed from the LB agar plate with PBS (10 ml, OD600nm = 0.6)

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

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