Protein Extraction and Western Blotting

Cells were collected and the total protein and nuclear protein were extracted according to the instructions (Beyotime, P0013B and P0027, respectively). The protein concentration was measured using a BCA protein determination reagent kit (Beyotime, P0011). Western blotting was performed as previously described^{50 (link)}. The primary antibodies included anti-GAPDH (YM1038) purchased from Immunoway; anti-LNGFR (ab8874) and anti-RunX2 (ab23981) purchased from Abcam; and anti-β-catenin (8480) and anti-Lamin B1 (13435) purchased from CST.

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Li G., Liu J., Wang Y., Yang K., Zhao M., Xiao Y., Wen X, & Liu L. (2017). LNGFR targets the Wnt/β-catenin pathway and promotes the osteogenic differentiation in rat ectomesenchymal stem cells. Scientific Reports, 7, 11021.

Publication 2017

P0027 anti-RunX2 (ab23981)

Anti b1 Antibodies Cells Gapdh Lamin Lamin b1 Nuclear protein Protein Runx2 β catenin

Corresponding Organization :

Other organizations : Army Medical University, Daping Hospital, Zunyi Medical University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Total protein
Nuclear protein
GAPDH
LNGFR
RunX2
β-catenin
Lamin B1

control variables

None explicitly mentioned

controls

Positive control: None mentioned.
Negative control: None mentioned.

Annotations

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