CENH3-GFP Transgenic Arabidopsis Lines

The CENH3-GFP reporter cassette driven by the different promotor sequences was constructed using the Multisite-Gateway cloning system according to manufacturer’s instructions (Invitrogen). Sequences of the primers used to clone the corresponding cDNA fragments are listed in Additional file 11: Table S2. Verified plasmids was transformed into the Agrobacterium tumefaciens strain GV3130 and used to generate transgenic Arabidopsis lines (ecotype Colombia-0) through floral dip methodology. Single T-DNA transgenic progeny plants were selected based on kanamycin resistance and stable GFP expression.

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De Storme N., Keçeli B.N., Zamariola L., Angenon G, & Geelen D. (2016). CENH3-GFP: a visual marker for gametophytic and somatic ploidy determination in Arabidopsis thaliana. BMC Plant Biology, 16, 1.

Publication 2016

Multisite-Gateway cloning system

Agrobacterium tumefaciens Arabidopsis Cdna Clone Ecotype Kanamycin resistance Plasmids Primers Strain Transgenic Transgenic plants

Corresponding Organization :

Other organizations : Ghent University

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Protocol cited in 16 other protocols

Variable analysis

independent variables

Promoter sequences

dependent variables

CENH3-GFP reporter cassette expression

control variables

Agrobacterium tumefaciens strain GV3130
Arabidopsis thaliana ecotype Colombia-0
Kanamycin resistance
Stable GFP expression

controls

Positive control: Kanamycin resistance
Positive control: Stable GFP expression

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