Genotyping was performed using the Illumina CardioMetaboChip v1.0 (Illumina, San Diego, CA) following the manufacturer’s protocol [21 (link), 22 (link)] in a total of 11,582 subjects using DNA purified by column from whole blood (Qiagen, Valencia, CA). The chip has been described elsewhere [4 (link)]. Calling of genotypes was performed using the Illumina Genome Studio software and both samples and SNPs were removed with call rates less than 98%.
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