TriVax Immunization Protocol for Antigen-Specific T-Cell Responses

TriVax immunization was used as previously described (Cho and Celis, 2009 (link)); mice were immunized intravenously (via tail vein injections) with Trp2_180–188 peptide or Trp2 and B8R_20–27 peptides, agonist-anti CD40 antibody (BioXCell), and vaccine-grade poly(I:C), a toll-like receptor 3 agonist (InvivoGen). Peptide doses of 50, 100, and 200 μg per mouse were used for effector time points, transfer experiments, and acute time points, respectively, unless otherwise noted. Animals that received TriVax immunization via intraperitoneal instead of intravenous injection were removed from the analysis unless otherwise noted.