Quantifying Pulmonary MMP Activity in TB

Lungs from M. tuberculosis-infected (14 wpi) and age-matched uninfected (control) mice were harvested and imaged in sealed transparent polycarbonate containers using an IVIS Lumina II imaging system (PerkinElmer, Waltham, MA) 24 h after intravenous injection of MMPSense^® 680 Fluorescence Imaging Agent (PerkinElmer), an in vivo imaging agent activated primarily by MMP-2, MMP-3, MMP-9 and MMP-13 (Jager et al., 2016 (link)). The data were analyzed using Living Image software (PerkinElmer).

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Ordonez A.A., Tasneen R., Pokkali S., Xu Z., Converse P.J., Klunk M.H., Mollura D.J., Nuermberger E.L, & Jain S.K. (2016). Mouse model of pulmonary cavitary tuberculosis and expression of matrix metalloproteinase-9. Disease Models & Mechanisms, 9(7), 779-788.

Publication 2016

IVIS Lumina II imaging system MMPSense® 680 Fluorescence Imaging Agent Living Image software

Lungs tuberculosis Mice Mmp 13 Mmp 2 Mmp 3 Mmp 9 Polycarbonate

Corresponding Organization :

Other organizations : Johns Hopkins University, Johns Hopkins Medicine, National Institutes of Health

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Variable analysis

independent variables

Infection status of mice: M. tuberculosis-infected (14 wpi) vs. age-matched uninfected (control)

dependent variables

Fluorescence signal from MMPSense® 680 Fluorescence Imaging Agent in the lungs of mice

control variables

Injection of MMPSense® 680 Fluorescence Imaging Agent
Lung imaging using IVIS Lumina II imaging system
Time of imaging (24 h after injection)

controls

Positive control: Lungs from M. tuberculosis-infected mice
Negative control: Lungs from age-matched uninfected (control) mice

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