Optimizing Protease Inhibitor Cocktail for Buccal Samples

Example 5

Optimizing Inhibitor Cocktail to Maximize Buccal Sample Non-Specific Protease Activity Inhibition

29 inhibitors were tested using a competitive enzymatic activity assay of 3CL protease in buccal samples.

PMSF, GW, aprotinin, eglinC and pepstatin all decreased non-specific protease activity in saliva, with a non significant inhibition of 3CL specific activity.

The four best inhibitors were Eglin C, GW, PMSF and 2,6 PDA.

Various inhibitor cocktails were also tested:

• • Cocktail 1 (PMSF, GW);
  • Cocktail 2 (Eglin C, GW, PMSF and 2,6 PDA)
  • Cocktail 2 reduced non-specific proteases to a greater degree than cocktail 1.