CHO-K1 (American Type Culture Collection [ATCC] CCL-61) cells and pgsA-745 (ATCC CRL-2242) cells, a derivative deficient in xylosyltransferase (UDP-D-xylose/serine-1,3-D-xylosyltransferase), referred to as CHO745 in this study, were maintained at 37 °C with 5% CO2 in Gibco F-12 Nutrient K (Kaighn’s Modification of Ham’s F-12) Medium (Thermo Fisher Scientific) supplemented with 10% HyClone Cosmic Calf Serum (Thermo Fisher Scientific) and 1× penicillin/streptomycin. The reverse genetics technique for the production of replication-competent, recombinant CedV was described previously (30 (link)). The rCedV antigenome plasmid was designed based on the sequence available in the GenBank (accession no. NC_025351.1).
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Reverse Genetics for Replication-Competent CedV
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Based sequence
Cells
Cosmic
Genetics of replication
Nutrient
Penicillin
Plasmid
Serum
Streptomycin
Xylose serine
Xylosyltransferase
Corresponding Organization :
Other organizations : Uniformed Services University of the Health Sciences, Mayo Clinic in Arizona, Memorial Sloan Kettering Cancer Center, Australian Centre for Disease Preparedness, National University of Singapore
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- Reverse genetics technique for the production of replication-competent, recombinant CedV
- Production of replication-competent, recombinant CedV
- CHO-K1 (American Type Culture Collection [ATCC] CCL-61) cells
- PgsA-745 (ATCC CRL-2242) cells, a derivative deficient in xylosyltransferase (UDP-D-xylose/serine-1,3-D-xylosyltransferase), referred to as CHO745 in this study
- Maintained at 37 °C with 5% CO2 in Gibco F-12 Nutrient K (Kaighn's Modification of Ham's F-12) Medium (Thermo Fisher Scientific) supplemented with 10% HyClone Cosmic Calf Serum (Thermo Fisher Scientific) and 1× penicillin/streptomycin
- Not explicitly mentioned
- Not explicitly mentioned
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