The dried CC (1 kg) was crushed and extracted twice with 10 L of 50% ethanol for 24 h. After filtration, the extracts were evaporated by rotary evaporator and freeze-dried at −70 °C. The extraction yield was 8% (w/w). All experiments on Chrysanthemum coronarium L. leaves were performed in accordance with the institutional, national, and international guidelines and legislation. CC (10 mg) was dissolved in 50% MeOH (10 mL), and the chemical profile of the extract was analyzed using UPLC-tunable UV (TUV) (Waters, Milford, MA, USA) with an Acquity UPLC BEH C18 column (100 × 2.1 mm, 1.7 μm; Waters). The eluted compounds were analyzed by Q-TOF/MS (Waters) in the negative electrospray ionization mode under optimized conditions as follows: a scan range of 50–1500 m/z, a scan time of 0.2 s, a capillary voltage of 2.5 kV, a sampling cone voltage of 40 V, a desolvation flow rate of 900 L/h, a desolvation temperature of 400 °C, source temperature of 100 °C, and sample injection volume of 1 μL. MS/MS spectra were acquired under collision energy ramp (10–30 eV). The major compounds were identified based on the online database UNIFI 1.8.2 (Waters) and other online databases, such as ChemSpider, Traditional Chinese Medicine database, and METLIN [16 (link)].
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