PBMC Immunophenotyping by Flow Cytometry

In this research, there were no more experiments performed. Immune staining and flow cytometry analyses have been performed in accordance with our previously published paper [7 (link)]. Briefly, peripheral blood mononuclear cells (PBMCs) were washed and suspended in flow cytometry staining buffer. Fc receptors (FcR) were first blocked using FcR Blocker (Miltenyi Biotec). Samples were then stained using particular antibodies that recognized cell surface or intracellular antigens. Samples were run on a BD LSRFortessa X-20 SORP flow cytometer, and data were collected using BD FACSDiva software (BD Biosciences), and then analyzed by FlowJo V10 software (FlowJo, Ashland, OR, USA).