PBS or Clodronate loaded liposomes (Liposoma B.V., Amsterdam, The Netherlands, ClodronateLiposomes.org) were injected retro-orbital at 10 ml/g mouse and animals sacrificed 36 h later10 (link). Fluorouracil (5-FU) (Sigma-Aldrich Corp., St. Louis, MO) was injected intraperitoneal at a dose of 150 mg/kg and animals sacrificed 4 days later81 (link).
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