Fasting blood samples were collected from participants at baseline from January 2010 to September 2011. Samples were centrifuged at 1000 g, for 15 min (4 °C). Serum and plasma were aliquoted and immediately stored at -80 °C until the day of the test in November and December of 2020. No freeze-thawing occurred during the storage.
Serum creatinine and cystatin C were measured by Roche Cobas 8000 modular analyzer Series (Roche, Inc., Mannheim, Germany) with a particle-enhanced immunonephelometric assay (Roche, Tina-quant Cystatin C, Mannheim, Germany) and the enzymatic assay creatinine PlusVer.2 (Roche, Mannheim, Germany) on Cobas c702 and Cobas c501 platforms, respectively [5 (link)].
Serum creatinine and cystatin C were measured by Roche Cobas 8000 modular analyzer Series (Roche, Inc., Mannheim, Germany) with a particle-enhanced immunonephelometric assay (Roche, Tina-quant Cystatin C, Mannheim, Germany) and the enzymatic assay creatinine PlusVer.2 (Roche, Mannheim, Germany) on Cobas c702 and Cobas c501 platforms, respectively [5 (link)].
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