Livers were fixed in 10% formalin overnight at 4°C, then gradually dehydrated with ethanol and embedded in paraffin. Immunohistochemistry and hematoxylin and eosin (H&E) staining were performed by the Texas Digestive Diseases Morphology Core at Baylor College of Medicine, as previously described.17 (link) The following primary antibodies were used for immunohistochemistry: rabbit anti 2A peptide (1:7500, ABS31, Sigma-Aldrich), rabbit anti Ki67 (1:60, CRM325, Biocare); rabbit anti FAH (1:65, ab151998, Abcam); rabbit anti Cd34 (1:250, ab81289, Abcam). The Ki67, FAH and Cd34 antibodies were then detected with a Rabbit-on-Rodent HRP-Polymer (RMR622H, Biocare) and visualized with DAB chromogen (DB801, Biocare). The 2A antibody was detected and visualized using a Leica Bond Polymer Detection kit (DS9800). Tunel staining was performed and detected using ApopTag Peroxidase In Situ Apoptosis Detection Kit (Millipore, S7100). Reticulin staining was performed using the Epredia Reticulin Sliver Stain Kit (87025, Epredia), following the manufacturer instructions. Slides were counterstained with hematoxylin, dehydrated, and mounted with a permanent mounting medium. A Nikon Ci-L bright field microscope was used for imaging at the Integrated Microscopy core (Baylor College of Medicine).