Spinal column was harvested from intranasal TK− HSV-2 immunized mice 45 min after tail vein injection with 200 µl of 5 mg/ml Oregon Green 488-conjugated dextran (70kDa, D7173, Thermo Fisher Scientific, MA) in PBS. Spine was then fixed with 4% paraformaldehyde in PBS overnight, and cut frozen sections (8 µm in thickness) for immunohistochemical analysis30 (link).
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Spinal Column Analysis of HSV-2 Immunity
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Other organizations : Howard Hughes Medical Institute
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Variable analysis
independent variables
- Intranasal TK⁻ HSV-2 immunization
- Tail vein injection of 200 µl of 5 mg/ml Oregon Green 488-conjugated dextran (70kDa, D7173, Thermo Fisher Scientific, MA) in PBS
- Spinal column harvested 45 min after tail vein injection
- Time (45 min) between tail vein injection and spinal column harvesting
- No positive or negative controls were explicitly mentioned in the input protocol.
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