Cell Viability Assay with CCK-8

HEI-OC1 cells were collected, washed after the treatment, and plated on a 96-well plate at a density of 10,000 cells/well. The CCK-8 reagent (40203ES76; YEASEN, China; 10 μL), which can undergo a reduction reaction with succinate dehydrogenase in the mitochondria of living cells, was added to HEI-OC1 cells for 4 h of incubation (conventional culture conditions). The 96-well plate was placed in a microplate reader to analyze the absorbance at 450 nm.

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Zhang X., Zheng J., Xu H, & Ma Z. (2023). UHRF1-induced connexin26 methylation is involved in hearing damage triggered by intermittent hypoxia in neonatal rats. Open Medicine, 18(1), 20230650.

Publication 2023

CCK-8 reagent

Cck 8 Cells Mitochondria Succinate dehydrogenase

Corresponding Organization : Tongji University

Other organizations : Shanghai East Hospital

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Variable analysis

independent variables

Treatment applied to HEI-OC1 cells

dependent variables

Mitochondrial activity (reduction of CCK-8 reagent) in HEI-OC1 cells, measured by absorbance at 450 nm

control variables

Cell density (10,000 cells/well)
Incubation time with CCK-8 reagent (4 hours)
Culture conditions (conventional)

controls

No positive control specified
No negative control specified

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