Live/Dead Cell Imaging Assay

Live and dead cell images were obtained using a Live/Dead^® Cell Imaging kit (488/570; R37601; Thermo Fisher Scientific) according to the manufacturer's instructions. Hydrolyzes the cell membrane permeable compounds that convert to the fluorescent anion calcein, thus detecting live cells. The dead cells were detected using ethidium homodimer (EthD-1) that stains the dead cells, which are impermeable to the dye, due to a compromised cell membrane. Images were obtained using a confocal laser scanning microscope (LSM 700; Carl Zeiss Microscopy Co., Ltd., Tokyo, Japan).

Free full text: Click here

Siddiqui S.H., Khan M., Park J., Lee J., Choe H., Shim K, & Kang D. (2023). COPA3 peptide supplementation alleviates the heat stress of chicken fibroblasts. Frontiers in Veterinary Science, 10, 985040.

Publication 2023

Live/Dead® Cell Imaging kit LSM 700

Anion Calcein Cell membrane Cell membrane permeable Cells Confocal laser scanning microscope Ethd 1 Ethidium homodimer Microscopy Stains

Corresponding Organization : Jeonbuk National University

Other organizations : The Barbara Ann Karmanos Cancer Institute, University of Rochester Medical Center, Wayne State University, Konkuk University

Top 5 similar protocols

Variable analysis

independent variables

Live/Dead^® Cell Imaging kit (488/570; R37601; Thermo Fisher Scientific)

dependent variables

Live cells detected using calcein fluorescence
Dead cells detected using ethidium homodimer (EthD-1) fluorescence

control variables

Manufacturer's instructions for Live/Dead^® Cell Imaging kit (488/570; R37601; Thermo Fisher Scientific)
Confocal laser scanning microscope (LSM 700; Carl Zeiss Microscopy Co., Ltd., Tokyo, Japan)

controls

Positive control: Live cells stained with calcein
Negative control: Dead cells stained with ethidium homodimer (EthD-1)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!