The Transwell migration assay was performed as described (21 (link)). A total of 5×104 MGC-803 or GES-1 cells were plated on the top chambers of 8 µm pore size Transwell plates (Corning Incorporated, Corning, NY, USA). In order to perform the Matrigel-coated Transwell invasion assay as described (21 (link)), Matrigel and 8×104 MGC-803 or GES-1 cells were plated on the top chambers of 8 µm pore size Transwell plates (Corning Incorporated, Corning, NY, USA). The migration and invasion assays were conducted for 48 h; all experiments were performed at least three times in triplicate.
Protocol detail
Transwell Migration and Invasion Assays
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Variable analysis
independent variables
- Cell type (MGC-803 or GES-1 cells)
- Cell migration
- Cell invasion
- Pore size of Transwell plates (8 µm)
- Duration of migration and invasion assays (48 h)
- Number of replicates (at least three times in triplicate)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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