After having been treated with dsGFP or dsCpr21L for 2 days, five nymphs of the 4th instar were sampled for each group and placed on ice, and their heads and thoraces were quickly removed with tweezers under a Nikon C-DSS230 stereomicroscope. The remaining part of the insect body without the head and thorax was quickly soaked in 2.5% glutaraldehyde solution and fixed overnight at 4 °C. After the samples were fixed well, subsequent processing was performed using a previously reported method [8 (link),23 (link)]. Semi-thin sections (2 μm) were cut using glass knives on an LKB Bromma 11,800 pyramitome (LKB, Bromma, Sweden) and stained with methylene blue. The ultra-thin sections were prepared with a diamond knife using PowerTome-PC (RMC, Boeckeler Instruments, Tucson, AZ, USA). The sections were stained with 3% uranyl acetate and alkaline lead citrate and were observed using a Hitachi H-7650 TEM (Hitachi, Tokyo, Japan).
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