The free thiol group of H-CREKA-NH2 peptide was used to form a thioether bond with the maleimide moiety of a functionalized fatty acid derivative (Supplementary Scheme S2B). Twenty mg of DSPE-PEG2000-Mal was dissolved in N2-purged PBS buffer (pH = 7), and the peptide compound (2 equiv.) was added into the solution in 10 equal portions under one hour. After a 20 h reaction time at RT, the compounds were separated by RP-HPLC. The fractions with conjugates were lyophilized before the MS characterization.
Vári B., Dókus L., Borbély A., Gaál A., Vári-Mező D., Ranđelović I., Sólyom-Tisza A., Varga Z., Szoboszlai N., Mező G, & Tóvári J. (2023). SREKA-targeted liposomes for highly metastatic breast cancer therapy. Drug Delivery, 30(1), 2174210.
Corresponding Organization : Eötvös Loránd University
Other organizations :
HUN-REN Research Centre for Natural Sciences, Institute of Materials and Environmental Chemistry, Országos Korányi Tbc és Pulmonológiai Intézet
Formation of thioether bond between free thiol group of H-CREKA-NH2 and maleimide moiety of DSPE-PEG2000-Mal
Yield of conjugated compounds after RP-HPLC separation
control variables
PH of the PBS buffer (pH = 7)
Reaction time (20 h)
Reaction temperature (RT)
Nitrogen purging of the PBS buffer
positive controls
Not explicitly mentioned
negative controls
Not explicitly mentioned
Annotations
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