RAD-seq for Resistant and Susceptible Lines

Leaves from plant materials listed in Table 1 were collected to extract DNA representing the material from SBSI. For the material from Strube Research, 70 plants from 5 extremely resistant lines (SR-R1, SR-R2, SR-R3, SR-R4, and SR-R5) and 110 plants from 5 extremely susceptible lines (SR-S1, SR-S2, SR-S3, SR-S4, and SR-S5) were selected for DNA extraction. Genomic DNA was obtained using a modified CTAB DNA extraction method [33 ]. The quality of the extracted DNA was checked on a 0.8% agarose gel and quantification of the same was done using a Qubit 4.0 fluorimeter (Thermo Fisher Scientific, Waltham, MA, USA). The DNA samples were subjected to a restriction site-associated DNA technology (RAD-seq) [34 (link)] with a HiSeq 2000 sequencing system using 150-bp single-end sequencing (Illumina Inc., San Diego, CA, USA).