The BrdU assay was performed according to the BrdU Cell Proliferation Assay Kit (Cat#: 6813, CST, MA, USA), as previously described [21 (link)]. CaOV3 and SKOV3 cells (5 × 103 cells) were seeded into 96-well plates and cultured overnight. After washing twice, the cells were denatured and labeled with 10 µL of 10X BrdU solution antibody for 2 h. Then, the medium was removed, and the HRP-conjugated secondary antibody solution was added into each well and incubated for 1 h. Finally, OD450 was performed on a multimode-plate reader (Thermo Fisher).
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