The antisera used were rat monoclonal anti-HA (Roche; catalog #11867423001), rabbit monoclonal anti-KIAA0310 (Thermo Fisher Scientific; catalog #A300-648A-M), mouse monoclonal anti-cis-Golgi matrix protein 130 (BD Biosciences; catalog #610823), mouse monoclonal anti-CNX (Sigma-Aldrich; catalog #C7617), mouse monoclonal anti-p230 (BD Biosciences; catalog #611281), sheep polyclonal anti-TGN46 (AbD Serotec; catalog #AHP500G), mouse monoclonal anti-β-actin (Sigma-Aldrich; catalog #A5441), mouse monoclonal ANTI-FLAG M2-peroxidase (HRP; Sigma-Aldrich; catalog #A8592), mouse monoclonal anti-EEA1 antibody (BD Biosciences; catalog #610457), rabbit polyclonal anti-Rab11 antibody (gift from the De Camilli laboratory, Yale School of Medicine, New Haven, CT, self-made), rabbit monoclonal anti-Lamp1 antibody (Cell Signaling; catalog #9091S), and mouse monoclonal anti-CatD antibody (BD Biosciences; catalog #610801).
Secondary antibodies were purchased from Thermo Fisher Scientific (catalog numbers A-11034, A-11029, A-21209, A-21208, A-21203, 32260, 31470, and 32230).
Anti-Cab45 antibody was generated by the animal facility (immunization service) of the Max Planck Institute of Biochemistry as described previously (Crevenna et al., 2016 (link)). Full-length recombinant Cab45 (His-Sumo tagged) was used for rabbit immunization.
Secondary antibodies were purchased from Thermo Fisher Scientific (catalog numbers A-11034, A-11029, A-21209, A-21208, A-21203, 32260, 31470, and 32230).
Anti-Cab45 antibody was generated by the animal facility (immunization service) of the Max Planck Institute of Biochemistry as described previously (Crevenna et al., 2016 (link)). Full-length recombinant Cab45 (His-Sumo tagged) was used for rabbit immunization.
