Protocol detail

Bioinformatics Analysis of Breast Cancer

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The TCGA database in UALCAN (http://ualcan.path.uab.edu) was used to validate the mRNA expression of the hub genes based on sample type and cancer stage. Significance was considered at a P value of 0.05. The Human Protein Atlas (HPA) (https://www.proteinatlas.org/) was used to explore the protein expression levels of hub targets in normal and BC tissues. The Kaplan‒Meier mapper database (http://kmplot.com/analysis/) was used to analyze the overall survival (OS) value of the mRNA expression of hub targets. The hazard ratio (HR) and log rank P value were calculated and displayed in the graph, and a log rank P value < 0.05 was set as a significant difference. The cBioPortal tool (http://www.cbioportal.org/) was used to discover the genetic information and the correlation between mRNA expression of core targets. A total of 818 breast invasive carcinoma samples (TCGA, Cell 2015) were analyzed. The genomic profiles of 10 hub targets were examined using mutations and putative copy-number alterations in the Genomic Identification of Significant Targets in Cancer (GISTIC) tool.

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Lv Y., Mou Y., Su J., Liu S., Ding X., Yuan Y., Li G, & Li G. (2023). The inhibitory effect and mechanism of Resina Draconis on the proliferation of MCF-7 breast cancer cells: a network pharmacology-based analysis. Scientific Reports, 13, 3816.

Publication 2023

Cancer Cancer stage Carcinoma of breast Cell Copy number alterations Expression genes Genomic Genomic profiles Hub protein Human protein Information Mrna Mutations Tissues

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Variable analysis

independent variables

MRNA expression of the hub genes
Protein expression levels of hub targets in normal and BC tissues
MRNA expression of hub targets

dependent variables

Sample type
Cancer stage
Overall survival (OS)

control variables

P value of 0.05 for significance
Log rank P value < 0.05 for significant difference in overall survival

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