Angiotensin peptides were measured in the adrenocortical tissues, in plasma collected during adrenal vein sampling (AVS) from each adrenal vein and from the infrarenal inferior vena cava. These measurements were blindly performed at Attoquant Diagnostics GmbH (Vienna, Austria) with liquid chromatography tandem-mass spectrometry analysis (LC-MS/MS), as described in detail [18 (link)]. The lower limits of detection for these assays are shown in Table 1 .
RAS equilibrium analysis was performed in human heparin plasma pool containing 50 ng/ml rhACE-2 (recombinant human ACE-2; R&D Systems, Vienna, Austria) in the presence and absence of 100 μg/ml DIZE (Sigma-Aldrich, Milan, Italy) in order to clarify if DIZE is an allosteric activator of ACE-2 as described [19 (link)].
RAS equilibrium analysis was performed in human heparin plasma pool containing 50 ng/ml rhACE-2 (recombinant human ACE-2; R&D Systems, Vienna, Austria) in the presence and absence of 100 μg/ml DIZE (Sigma-Aldrich, Milan, Italy) in order to clarify if DIZE is an allosteric activator of ACE-2 as described [19 (link)].
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